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. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: J Immunol. 2012 May 11;188(12):6371–6380. doi: 10.4049/jimmunol.1103527

Figure 4. EVI1 negatively regulates bacteria-induced NF-κB activation via direct interaction with p65.

Figure 4

(A & B) A549 cells co-transfected with Flag-EVI1 and p65 were treated with NTHi for the times indicated in the figure, and the proteins were then immunoprecipitated with anti-Flag antibody (A) or anti-p65 antibody (B). Immunoprecipitations were then analyzed by western blot analysis with the indicated antibodies. (C) A549 cells co-transfected with Flag-EVI1 and p65 were treated with NTHi and then triple-immunostained with DAPI, anti-p65 and anti-Flag antibodies. Slides were visualized by fluorescence microscopy. (D) A549 cells were treated with NTHi or TNF-α for the times indicated in the figure, and the proteins were then immunoprecipitated with anti-p65 antibody and analyzed by western blot analysis with the indicated antibodies. Data are representative of at least three independent experiments.