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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: J Immunol. 2012 May 11;188(12):5906–5914. doi: 10.4049/jimmunol.1103233

Figure 4.

Figure 4

C/EBPs are essential for a subset of IL-17-induced genes. A) Real-time PCR analyses for indicated genes with RNAs isolated from WT, C/EBPβ deficient (βKO), C/EBPδ deficient (δKO) and C/EBPβδ doubly deficient (βδKO) MEFs stimulated for 1h or 6h with IL-17, TNFα, IL-17 + TNFα. Data are shown as the mean ± SEM of four independent experiments and fold induction is in reference to unstimulated cells; *, p < 0.05 Student’s t-test. B) Western blot analysis of cell lysate collected from a representative experiment shown in (A) after 2h of IL-17 stimulation to confirm absence or show expression levels of C/EBPβ and C/EBPδ; β-actin served as a loading control.