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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: J Immunol. 2012 May 14;188(12):6119–6126. doi: 10.4049/jimmunol.1200570

Figure 4.

Figure 4

15 kDa granulysin activates human monocytes. A, Purified CD14+ monocytes were cultured in medium alone or in medium supplemented with 50 uM 9 kDa granulysin, 10 nM 15 kDa granulysin or 10 ng/ml GM-CSF. Photographs were taken (10X objective) 6 hours later. B, Expression of a panel of cell surface molecules by monocytes cultured with 15 kDa granulysin (10 nM) or GM-CSF (10 ng/ml). Purified monocytes were incubated with medium (grey), 10 nM 15 kDa granulysin (blue) or 10 ng/ml GM-CSF (orange) for 24 hours and then stained with fluorescent antibodies and analyzed by FACS. C, iDC were incubated with 10 nM 15 kDa granulysin (black line) or medium (grey) for an additional 24 hours and then stained with the indicated fluorescent antibodies and analyzed by FACS. Results are representative of 4–6 similar experiments.