FIGURE 3.

Activation of human p21 promoter by TLP. A, luciferase reporter assay for various human promoters. HeLa cells transfected with the indicated reporter plasmids together with FH-TLP or its vacant plasmids (vector) were examined for relative luciferase activity using the Dual-Luciferase Reporter Assay System. B, schematic illustration of human genomic DNA around the p21 gene from −2677 to +66 inserted in p21luc1 and its derivative plasmids. p21luc1 contains transcription start sites at −2230 for the upstream promoter and +1 for the downstream promoter. p21luc3 is a promoterless construct. Solid boxes represent reported p53-binding sites. C, COS7 cells were transfected with increasing amounts of FH-TLP, and luciferase activity derived from the p21 promoter in p21luc2 and BAX promoter was measured. Data are indicated as averages with error bars according to multiple experiments.