FIGURE 2.

Dynamin protects K562 cells from complement-dependent cytotoxicity. K562 cells were transfected with the K44A plasmid or an empty vector control (EGFP) for 24, 48 or 72 h (A) or for 30 min at 37 °C with Dynasore at different concentrations (B). Then, the cells were subjected to treatment with an anti-K562 antiserum (diluted 1/18 or 1/22) followed by complement for 60 min at 37 °C. Cell lysis was determined by measuring uptake of trypan blue. Results are representative of four independent experiments, *, p < 0.05; **, p < 0.01.