FIGURE 6.

Caveolin-1 contributes to MAC elimination by exo-vesiculation. K562 cells were transfected with a Cav-1-EGFP or Cav-1-DGI plasmid or with an empty vector as control. After 48 h the cells were treated with a sublytic dose of anti-K562 antiserum and then with NHS for 10 min at 37 °C. The cells were extensively washed with HBSS and incubated at 37 °C for 5 or 10 min. The cell supernatant (sup) was collected and cleared by centrifugation at 5,000 × g, subjected to SDS-PAGE and analyzed by Western blotting with anti-C9 antibodies. A, representative C9 protein bands in sup. B, results of densitometric analyses of protein bands from three independent experiments.