FIGURE 1.

Localization of SbdP in A. aeolicus. A, immunogold labeling of SbdP on ultrathin sections of A. aeolicus. White arrows point to cytoplasmic SbdP, and the black arrow represents membrane-associated SbdP. The bar represents 200 nm. B, 30 μg of solubilized (sol) membranes (1st lane), solubilized NaBr-washed membranes (2nd lane), NaBr supernatant (3rd lane), or “cytoplasm” (4th lane) were loaded onto denaturing reducing gel, and SbdP (12.8 kDa) was detected using specific antibodies. The molecular mass markers are indicated in kDa on the left. C, rhodanese activity of solubilized membranes (column 1), solubilized NaBr-washed membranes (column 2), and NaBr supernatant (column 3). 100% represents the maximal rhodanese activity measured in the membrane fraction (200 μmol·min⁻¹, specific activity = 4.8 μmol·min⁻¹·mg⁻¹) and corresponds to 10% of the total rhodanese activity. The results shown are averages of three independent experiments.