FIGURE 2.

Analysis of solubilized membranes from cross-linked intact cells. A, native gels loaded with heavy fraction of solubilized membranes from sucrose gradient. Lane 1, Hydrogenase activity; lane 2, SbdP detection via Western blot. The arrow indicates the position of SbdP and hydrogenase in the gel. B, protein identification of the band sliced from the native gel (marked with a box) using ion trap MS analysis. Only proteins identified with at least five unique peptides and involved in energy electron transfers are included in this table. Table headings are as follows: protein name, name in NCBI data base; NCBI entry, accession number; coverage %; protein sequence coverage by the matching peptides; total peptides, number of total peptides matching to protein sequence; unique peptides, number of different peptides matching the protein sequence.