Preimplantation development of ICSI, ESNT, CCNT, and MEFNT embryos. Reconstructed embryo number was considered as 100% in each group. 4-Cell, morula, and blastocyst development rates were calculated based on 2-cell embryo numbers. ICSI embryos had the highest development rate at 4-cell, morula, and blastocyst stages. MEFNT embryos had significant lower development rates when compared with other groups at different stages and showed a dramatic drop from morula to blastocyst stage.