FIGURE 5.

Changing of B23 signal in NT and ICSI embryos during a 1-h post-NT/sperm injection. B23s were labeled by monoclonal anti-UBF antibody and fluorescent conjunct second antibody (green). The donor cell chromatin was stained with Hoechst 33342 (blue). Arrowheads indicate the donor cell chromatin/sperm head. The B23 signals in CCNT and MEFNT were showed as several obvious clusters (I and M). But after 20 min, the signals got much weaker (J and N). No B23 signal could be detected after 40 min (K, L, O, and P). The B23 in ESNT group lasted for 60 min, although the signal got weaker over time, but the signal was not companied with chromatins (E–H). No B23 signal could be detected at sperm head or maternal chromatins (A–D).