FIGURE 2.

Association with galectin lattice affects FGF21-stimulated phospho-ERK1/2 response. Prior to stimulation (100 ng/ml FGF21), HeLa cells co-transfected with KLB-Venus and R1-Cerulean were cultured in the presence or absence of lactose (20 mm; 48 h) and serum-starved (0.2% FBS) overnight. Cells were then stimulated with 100 ng/ml FGF21 (time points indicated) and harvested for Western immunoblot analysis. A and B, representative phospho-ERK1/2 (pERK1/2) and total ERK1/2 immunoblots (A) and the mean -fold phospho-ERK1/2 responses (B) revealed an earlier and more robust response to FGF21 after lactose treatment to dissolve the galectin lattice. Data are plotted as the mean -fold phospho-ERK1/2 response ± S.E. of five separate samples.