Aggregation state of R1-Venus, KDR1-Venus, and KLB-Venus in unstimulated cells.
A, R1-Venus, KDR1-Venus, and KLB-Venus were co-expressed with their Cerulean counterparts at the plasmid mass ratio indicated. R1-Venus and KDR1-Venus showed a 2-fold decrease in relative brightness (ρ) after expressing their Cerulean counterparts. In contrast, KLB-Venus showed no difference after co-expression of KLB-Cerulean. The data are plotted as the mean ± S.E. for 12 or more cells. * and # indicate a p value of < 0.05 by Tukey's honestly significant difference test after one-way analysis of variance. B, the relative brightness (ρ) of KLB-Venus, R1-Venus, and KDR1-Venus after co-expression with the indicated receptor. After co-expression with KLB-Cerulean, the brightness of R1-Venus decreased almost 2-fold.