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. 2012 Apr 24;287(24):20016–20026. doi: 10.1074/jbc.M112.344077

FIGURE 7.

FIGURE 7.

Simulated voltage- and substrate-dependent conformational changes, open probabilities and transport currents of cysteine-substituted and D83A EAAT3. A–F, averaged voltage dependence of measured late fluorescence changes (fluorescence-voltage relationship) (circles) and simulated fluorescence changes (solid lines) in the absence (gray) or in the presence of 1 mm glutamate (black). Fluorescence amplitudes were normalized to the fluorescence generated at +150 mV in NaCl solution. G and H, simulated open probabilities (G) at +100 mV of cysteine-substituted WT and D83A EAAT3 without (gray) and in the presence of glutamate (black) and simulated transport currents (H) at −100 mV from the data given in Tables 1 and 3. e, elementary change; e = 1.602 176 462(63)·10−19c. I and J, simulated residence probability of cysteine-substituted WT (I) and D83A EAAT3 (J) calculated from the data given in Table 1 without (gray) and in the presence of glutamate (black) for three different voltages, −150 (solid line), 0 (dashed line), and +150 mV (dotted line). glu, glutamate; F, fluorescence.