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. 2012 Apr 17;287(24):20027–20036. doi: 10.1074/jbc.M111.334953

FIGURE 4.

FIGURE 4.

Effect of disease-related protein modifications, correcting TnC and their combinations on rate of Ca2+ dissociation from thin filament. A, the time courses of the decrease in IAANS fluorescence as Ca2+ was removed by EGTA from thin filament-bound control TnIAANST53C, TnI R192H TnIAANST53C, TnC S69D TnIAANST53C, and TnI R192H-TnC S69D TnIAANST53C. B, the time courses of the decrease in IAANS fluorescence as Ca2+ was removed by EGTA from thin filament-bound control TnIAANST53C, TnT ΔK210 TnIAANST53C, TnC M45Q/S69D TnIAANST53C, and TnT ΔK210-TnC M45Q/S69D TnIAANST53C. C, the time courses of the decrease in IAANS fluorescence as Ca2+ was removed by EGTA from thin filament-bound control TnIAANST53C, ischemic TnI(1–192) TnIAANST53C, TnC S69D/D73N TnIAANST53C, and TnI(1–192)-TnC S69D/D73N TnIAANST53C. The data traces have been staggered and normalized for clarity.