Engineered TnC constructs functionally correct DCM TnT ΔK210 and ischemic TnI-(1–192) skinned cardiac muscle force generation.
A, the Ca2+-dependent force generation for skinned trabeculae exchanged with control TnIAANST53C (□), TnT ΔK210 TnIAANST53C (▿), and TnT ΔK210-TnC M45Q/S69D TnIAANST53C (▾). B, the Ca2+-dependent force generation for skinned trabeculae exchanged with control TnIAANST53C (□), TnI-(1–192) TnIAANST53C (▵), and TnI-(1–192)-TnC S69D/D73N TnIAANST53C (▴). Data traces were individually normalized.