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. 2012 Apr 17;287(24):20027–20036. doi: 10.1074/jbc.M111.334953

TABLE 2.

Summary of actomyosin ATPase activity

Values marked with* are significantly different from the control values (p < 0.05).

Tn ATPase pCa50a ATPase Ca2+ sensitivity Hill coefficient Maximal ATPase activityb Minimal ATPase activityc
μm
Control 5.81 ± 0.03 1.6 ± 0.1 1.18 ± 0.09 0.037 ± 0.003 0.0072 ± 0.0006
TnI R192H 6.29 ± 0.03* 0.52 ± 0.04* 1.1 ± 0.1 0.029 ± 0.003 0.0110 ± 0.0008*
TnC S69D 5.27 ± 0.07* 5.6 ± 0.9* 1.0 ± 0.2 0.035 ± 0.003 0.010 ± 0.002
TnI R192H-TnC S69D 5.84 ± 0.09 1.5 ± 0.3 0.83 ± 0.08* 0.038 ± 0.002 0.012 ± 0.001*

a The Ca2+ concentration at half-maximal ATPase activity.

b Maximal ATPase activity [mol of phosphate s−1 (mol of S1)−1].

c Minimal ATPase activity [mol of phosphate s−1 (mol of S1)−1].