FIGURE 5.
PKCδ inhibits the association of MALT1 with TRAF6. A, PKCδ disrupts the interaction of MALT1-TRAF6, but not CARMA1-Bcl10, CARMA1-MALT1, and Bcl10-MALT1. The expression plasmids were cotransfected in HEK293 cells as indicated. Immunoprecipitation and immunoblots were performed using the indicated antibodies. B, knockdown of PKCδ by siRNA accelerates the recruitment of TRAF6 to MALT1 upon CD3/CD28 costimulation. PKCδ-knockdown Jurkat T cells and controls (5 × 107 each) were stimulated by CD3/CD28 crosslinking for the indicated time. Cell lysates were subjected to immunoprecipitation with an antibody to MALT1. Immunoprecipitates and lysates were immunoblotted using the indicated antibodies.