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Purification of Msp from L. pneumophila culture supernatant
AU means arbitrary unit.
| Material | Volume | Protein | Total protein | Total activity | Recovery | Specific activity |
|---|---|---|---|---|---|---|
| ml | μg/ml | mg | AU | % | AU/μg | |
| Culture supernatant | 3100 | 3384 | 10,490 | 1.4 × 108 | 13.35 | |
| Desalted precipitate | 22 | 975 | 21.44 | 8.16 × 107 | 58 | 3806 |
| HiPrep DEAE FF 16/10 | 120 | 42 | 5.05 | 1.1 × 108 | 134 | 21,825 |
| HiLoad 16/60 Superdex 75 | 20 | 75 | 1.50 | 6.0 × 107 | 54 | 40,000 |
