FIGURE 2.

Impact of empedopeptin on macromolecular biosyntheses of B. subtilis 168. Untreated (triangles), empedopeptin-treated (squares), or vancomycin-treated (open circles) cells (both 8× MIC) were incubated with tritium-labeled precursors to monitor their incorporation into proteins (A), DNA (B), RNA (C), and cell wall (D). [³H]Phenylalanine was used to quantify protein synthesis, [³H]thymidine was used to quantify DNA synthesis, [³H]uridine was used to quantify RNA synthesis, and [³H]GlcNAc was used to quantify cell wall synthesis.