TABLE 2.
Antagonistic effect of peptidoglycan and wall teichoic acid precursors and truncated variants thereof on antimicrobial activity of empedopeptin
Empedopeptin at 8× MIC was exposed to the potentially antagonizing agents in the indicated molar ratios prior to mixture with B. subtilis in culture broth containing 1.25 mm Ca2+. Results of two independent experiments are summarized. +, antagonization; −, no antagonization.
| Lipid intermediate | Molar ratio of precursor to empedopeptin |
||||||
|---|---|---|---|---|---|---|---|
| 10× | 5× | 2.5× | 1.25× | 0.625× | 0.3125× | 0.156× | |
| UDP-GlcNAc | − | − | − | − | − | − | − |
| UDP-MurNAc-pp | − | − | − | − | − | − | − |
| C55-P | − | − | − | − | − | − | − |
| C15-PP | + | + | + | − | − | − | − |
| C55-PP | + | + | + | − | − | − | − |
| Lipid I(dipeptide) | + | + | + | + | + | − | − |
| Lipid I(tripeptide) | + | + | + | + | + | − | − |
| Lipid I(pentapeptide) | + | + | + | + | + | + | − |
| Lipid II | + | + | + | + | + | + | − |
| Lipid III | + | + | + | + | − | − | − |