TABLE 3.

Initial rates of RNA unwinding

	5′-30-nt ds10		3′-30-nt ds10		ds10
	Unwinding ratea	Amplitude	Unwinding ratea	Amplitude	Unwinding ratea	Amplitude
eIF4A	9.5 ± 0. 7	0.46 ± 0.04	6.5 ± 0.81	0.22 ± 0.02	1.2 ± 0.22	0.16 ± 0.02
WT eIF4F	113 ± 17.5	0.49 ± 0.05	4.9 ± 0.3	0.21 ± 0.04	4.7 ± 0.61	0.05 ± 0.02
			(4.7 ± 1.4)b	0.23 ± 0.05
WT eIF4Fc	67 ± 3.4	0.20 ± 0.01	NDd		ND
WT eIF4Fe	10.6 ± 1.1	0.40 ± 0.02	4.3 ± 1.2	0.18 ± 0.02	ND
eIF4F ΔRNA1	40 ± 5.5	0.55 ± 0.03	18 ± 2.4	0.61 ± 0.01	3.4 ± 0.3	0.15 ± 0.02
eIF4F ΔRNA2	9.9 ± 0.65	0.81 ± 0.05	13 ± 2.7	0.84 ± 0.05	7.3 ± 0.4	0.23 ± 0.02
eIF4F ΔRNA3	6.3 ± 0.23	0.83 ± 0.4	11.4 ± 0.9	0.88 ± 0.06	1.8 ± 0.2	0.06 ± 0.01

^a Data are (×10⁻³) min⁻¹.

^b Measured with the 3′-5 seq-30-nt-ds10 substrates (see Table 1).

^c Measured with capped RNA.

^d ND means not determined.

^e Measured with the 15-nt-ds10 substrates (see Table 1); all the errors shown are mean deviations; n ≥ 2.