FIGURE 6.
Increased β-cell proliferation in Gαz-null islets from mice fed the HFD as compared with their wild-type counterparts. A–D, pancreas sections from wild-type control diet-fed mice (A), Gαz-null control diet-fed mice (B), wild-type HFD-fed mice (C), and Gαz-null HFD-fed mice (D) were incubated with primary antibodies against insulin (red), glucagon (blue), and Ki67 (green). Overlaying the images revealed β-cells that are actively replicating (examples indicated by white arrows). E, quantification of the Ki67-positive β-cells as a percentage of the total β-cells. Three nonconsecutive sections separated by at least 100 microns were quantified for each pancreas. n = 2 mice for each group. The data were analyzed by unpaired t test. **, p < 0.01; ***, p < 0.001. ctrl, control.