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. 2012 Apr 13;287(24):20356–20368. doi: 10.1074/jbc.M112.349738

FIGURE 4.

FIGURE 4.

Deletion of the floxed Notch sequences in the cerebral cortex of Notch1 and Notch2 cKO mice. A and B, PCR using genomic DNA isolated from the neocortex and the hippocampus as well as the tail of Notch1 cKO (A) and Notch2 cKO (B) mice is shown. PCR was performed using three primers that can amplify and distinguish the floxed, deleted, and wild-type alleles. Only one PCR product (500 bp for the floxed Notch1 allele, 383 bp for the floxed Notch2 allele) is produced in the DNA samples from the neocortex, the hippocampus, and the tail of control (fNotch1/fNotch1 for Notch1 cKO, fNotch2/fNotch2 for Notch2 cKO) mice. In cKO mice, one PCR product (500 bp for the floxed Notch1 allele, 383 bp for the floxed Notch2 allele) is produced in the tail DNA sample, whereas two PCR products representing the floxed and the deleted (600 bp for Notch1, 489 bp for Notch2) alleles are produced in DNA samples from the neocortex and hippocampus, suggesting mosaic cell populations (some carrying the floxed allele, some carrying the deleted allele) in the cortex.