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Comparison of growth curves of Cac WT and AspRS2 (CAC3564, aspS2) knock-out strains. The strains were grown on minimal medium with ammonium as the only nitrogen donor and in the presence (+Asn) and absence of Asn (−Asn). Cell density was optically measured at 600 nm. Error bars = mean growth (OD)/time of three independent growth measurement replicates ± S.D. The Standard Deviation (S.D.) was less than 5%.
