TABLE 1.

Transposon insertion sites of mutants affected in PE_PGRS secretion

Gene	Annotation	(Putative) function	Mutant	PE_PGRS secretion on filtera	Colony morphologyb	Insertion in gene (bp 5′)	Orientation transposonc
mmar_1008		Conserved hypothetical membrane protein	B5	+/−	S	437	NT
mmar_2307		Hypothetical transmembrane protein	M168	−	R	1000	NT
mmar_2313	losA	Involved in assembly of the LOS-IV glycolipid	B12	+/−	S/W	88	NT
			B1	+/−	S/W	349	NT
mmar_2319		Conserved hypothetical transmembrane protein	B9	+/−	S/W	1548	+
			B10	+/−	S/W	223	−
mmar_2320	wecE	Pyridoxal phosphate-dependent enzyme	A10	+/−	S/W	839	−
			A8	+/−	S/W	755	−
			A3	+/−	S/W	215	+
mmar_2327	–	Conserved hypothetical transmembrane protein	B11	+/−	S	145	NT
			B2	+/−	S	773	NT
mmar_2332	ilvB1_3	Acetolactate synthase	B14	+/−	S/W	1683	+
mmar_2336	galE6	UDP-glucose 4-epimerase	B7	+/−	S	283	NT
mmar_2340	pks5	Polyketide synthase	B16	−	R	1051	−
			A12	−	R	2141	−
			A2	−	R	3265	−
mmar_2341	fadD25	Fatty acyl AMP ligase	B4	−	R	330	−
			A4	−	R	373	+
			B13, B15d	−	R	798	−
mmar_2353		UDP-glycosyltransferase	A1	−	R	751	NT
			A7	−	R	103	−
mmar_2355	papA3	Conserved polyketide synthase-associated protein	A5	−	R	691	+
			B8	−	R	139	−
			B3	−	R	120	+
mmar_2405	cphB	Cyanophycinase	A6, A11d	−	R	496	+
mmar_4419		Conserved hypothetical protein	B17	+/−	S	373	NT
mmar_5170	whiB4	Transcriptional regulatory protein Whib-like	A9	−	R	38	−

^a +/− indicates reduced, and − indicates negative.

^b S indicates smooth; R indicates rough; and S/W indicates smooth-wrinkled.

^c Orientation of the transposon is as follows: +, promoter activity in the same direction as the tested gene; −, promoter activity against the direction of the gene; NT, not tested.

^d Mutants were detected with a transposon insertion at the exact same location in the gene.