FIGURE 5.
Increased levels of Aβ1–40 and altered TCA cycle metabolism in MCK-βAPP muscle. A, ELISA measurement of the levels of human Aβ1–42 and Aβ1–40 in hamstring muscle extracts from MCK-βAPP mice in 30% formic acid. B, metabolic flux of 13C label from 1-[13C]pyruvate into isotopomers of lactate at C3, glutamate at C4 and C2, and succinate at C2/C3 as measured by [1H-decoupled]13C NMR spectroscopy. C, total metabolite pool size of lactate, glutamate, and succinate as measured by [13C-decoupled]1H NMR spectroscopy (n = 4; *, p < 0.05, t test).