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Effects of Ser-81 mutation on in vitro CK2-induced CaM phosphorylation. Shown is an autoradiograph of WT and mutant CaM phosphorylated with [γ-32P]ATP in the presence of CK2. Purified WT and mutant CaM were phosphorylated with CK2 as described in Methods, and this phosphorylation reaction was analyzed by SDS/PAGE and autoradiography. Each lane represents an independent phosphorylation reaction.
