Fig. 5.

Effects of CaM phosphorylation on eNOS enzymatic activity. (A) Enzymatic activity assay of immunopurified eNOS at various concentrations of CaM or phosphorylated CaM. eNOS was immunoprecipitated from BAEC with an anti-eNOS antibody. Purified preparations of vehicle- or CK2-treated CaM were added at various concentrations to the immune complexes, and the NOS activity was measured as described in Methods. The concentrations of CaM and phosphorylated CaM were determined by amino acid analysis. (B) A double reciprocal plot of the data in A.