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Effects of phosphomimetic CaM mutants on eNOS enzymatic activity. Shown are the results of enzymatic activity assays of immunoprecipitated eNOS at various concentrations of purified WT or mutant CaM in which putative phosphorylation sites were changed to aspartate. eNOS was immunopurified from BAEC with an antibody directed against eNOS. Various concentrations of purified WT or mutant CaM were added to the immune complexes, and NOS activity was measured as described in Methods.
