FIGURE 3.
TOCA1 overexpression enhances pedestal assembly while FBP17 overexpression inhibits it. A, COS7 cells co-expressing HA-Tir-EspFU(R1–6) and GFP fusion proteins were infected with E. coli expressing intimin, fixed, and stained with DAPI to identify bacteria and phalloidin to label F-actin. The scale bar is 1 μm in length. B, pedestal formation efficiencies were determined by calculating the % of infected GFP-expressing cells harboring 10 or more actin pedestals. Data represent the mean ± S.E. from 3–4 experiments. Pedestal intensities in relative fluorescent units (RFU) were determined by measuring the total F-actin pixel intensity in the pedestal. Data represent the mean ± S.E. from ∼70 pedestals per sample across 3–4 experiments. For comparisons to GFP: *, p < 0.05; ***, p < .0001.