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. 2012 Apr 27;287(24):20613–20624. doi: 10.1074/jbc.M112.363473

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — TOCA1 and EspF_U activate N-WASP/WIP additively in vitro. A, actin (2 μm) was polymerized using 20 nm Arp2/3 complex, 20 nm N-WASP/WIP complex, and the indicated concentrations of His-tagged EspF_U(R1–6), TOCA1 wild type, or the W518K mutant. Barbed end concentrations were calculated when F-actin reached its half-maximal value. B, actin (2 μm) was polymerized using 20 nm Arp2/3 complex, 20 nm N-WASP/WIP complex, and the indicated concentrations of TOCA1 and EspF_U(R1–6). Pyrene-actin fluorescence was measured in arbitrary units (AU) over time. C, actin (2 μm) was polymerized as in part B, and the time to reach half of the maximal F-actin concentration (Halftime) and slope of assembly curves at 60% polymer (Assembly Rate) was measured and normalized to controls containing only actin, Arp2/3 complex, and N-WASP/WIP complex. The predicted Halftimes and Assembly Rates were calculated based on the premise that the effects of TOCA1 and EspF_U on actin polymerization would be additive.