Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Apr 25;287(24):20652–20663. doi: 10.1074/jbc.M111.327601

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Depletion of NDUFS4 changes the assembly status of CI. A, representative BN-polyacrylamide gel showing crippled CI monomer when Ndufs4^fky/fky heart mitochondria are solubilized in 1% n-dodecyl β-maltoside (DDM; lane 2). When Ndufs4^fky/fky heart mitochondria are solubilized in 1% Triton X-100 (TX100), the CI-CIII₂ supercomplex (SC) can be observed (lanes 3 and 4). A large proportion of this CI-CIII₂ SC is detected in the crippled state in Ndufs4^fky/fky mitochondria (lane 4). B, BN-PAGE followed by Western blot with antibody against CI subunit NDUFA9 shows weaker staining and crippled status of CI (CI) monomer and CI-CIII₂ (CI/CIII₂) supercomplex in 1% Triton X-100-solubilized mitochondria from heart tissues of Ndufs4^fky/fky mice.