FIGURE 5.
Schematic representation of the results from nano-LC-MS/MS analysis of the crippled CI in mitochondria from Ndufs4fky/fky mice. Left, the different modules present in CI in wild type mice. Right, crippled complex I from Ndufs4fky/fky mice misses all subunits that make up the N module, whereas the assembly factors Ndufaf1 and Ndufaf2 were found associated with the crippled complex I. Representation of the outcome of nano-LC-MS/MS analysis was based on the assembly model described by McKenzie and Ryan (64).