FIGURE 6.
NDUFS4 depletion results in significant changes in blood metabolite levels as measured by tandem MS. A–C, tandem MS analysis of blood from dried bloodspot cards showed significant increases in hydroxyacylcarnitine species with a mass corresponding to hydroxy-C4:0 acylcarnitine, hydroxy-C16:0 acylcarnitine, and hydroxy-C18:1 acylcarnitine in Ndufs4fky/fky mice. D–F, the only amino acid levels that were significantly changed were those of glycine, phenylalanine, and homocitrulline (all increased). n = 27 for controls; n = 26 for Ndufs4fky/fky. Error bars, S.E.