Figure 4.

Altered Expression of Circadian Genes in Rai1^+/− Mouse Hypothalamus during Light and Dark Phases

(A) Circadian-gene expression in mouse hypothalamus extracted from WT (black bars) or Rai1^+/− mice (white bars) during the light phase at 8 hr Zeitgeber time.

(B) Circadian-gene expression in mouse hypothalamus extracted from WT (black bars) or Rai1^+/− mice (white bars) during the dark phase at 16 hr Zeitgeber time. All values shown are the mean of at least three independent samples analyzed in triplicate. The quantification of the test gene is compared to that of the housekeeping gene Gapdh. Error bars represent the standard deviation from the mean. Asterisks indicate p < 0.05 by Student's t test.