Figure 4.

P^R and P^S Yeast Phenotypes

(A) Spot assay of mto1Δ P^R and P^S strains, transformed with MTO1^WT recombinant vector, (empty) vector, and mto1^Ala431Thrand mto1^Pro622∗ recombinant vectors. The assay was performed by spotting decreasing concentrations of yeast cells (10⁵, 10⁴, 10³, and 10²) on a medium supplemented with either 2% glucose (upper panel) or 2% glycerol (lower panel). See text for details.

(B) Respiratory activity of yeast MTO1^WT and mutant P^R strains at 28°C. Respiratory rates were normalized to the WT strain, for which the respiratory rate was 26.6 nmol min⁻¹ mg⁻¹. Values are the mean of at least four independent experiments. Two-tail, unpaired t test was applied for statistical significance. ^∗: p < 0.05; ^∗∗p < 0.01; ^∗∗∗: p < 0.001.

Data are represented as mean ± SD.