Table 1.
Primers for PCR and real-time PCR
| Primer name* | Sequence (5′–3′) |
| Hsc70-dF | CARCAYGGCAARGTGGAGATCAT |
| Hsc70-dR | TCAATACTGGCYTGDGTGCT |
| Q-RT | CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGC(T)17 |
| Q-O | CCAGTGAGCAGAGTGACG |
| Q-I | GAGGACTCGAGCTCAAGC |
| Hsc70-3O | AGAAGGTTGGAGCGGAAAGGAATGT |
| Hsc70-3I | TCCCACCAAGCAGACTCAGACATTCA |
| Hsc70-5RT | TGCCACGGAACAGATCAGCATTTAA |
| Hsc70-5O | CCATACGGTTATCAAAGTCCTCCCCA |
| Hsc70-5I | ACATTCCTTTCCGCTCCAACCTT |
| Universal primer mix (UPM) | Long: CTAATACGACTCACTATAGCAAGCAGTGGTATCAACGCAGAGT |
| Short: CTAATACGACTCACTATAGC | |
| Nested universal primer (NUP) | AAGCAGTGGTATCAACGCAGAGT |
| Hsc70-ORF-F# | CCCAAGCTTATGTCTAAGGGACCAGCA |
| Hsc70-ORF-R§ | CCGCTCGAGATCTACTTCCTCAATGGTT |
| β-actin-qF | CTACAGCTTCACCACCACAG |
| β-actin-qR | GACCATCAGGGAGTTCGTA |
| Hsc70-qF | ATGAAGCTGTGGCTTACGGTGCA |
| Hsc70-qR | TGTTCCGCTTGATCAGGACTGTCA |
*
Last letters in the primer names: F, forward; R, reverse; RT, reverse transcription; O, outer; I, inner; qF, forward primers for quantitative PCR; qR, reverse primers for quantitative PCR
#
Underline means HindIII restriction endonuclease site
§
Underline means XhoI restriction endonuclease site