Fig. 1.

Sampling for expression microarray analysis from continuous synchrony cultures. Respiratory-reductive synchrony is monitored by using DO levels in the media of aerobic continuous cultures. The sample times are shown imposed on the DO oscillation. High DO levels are associated with the reductive phase and low DO levels with the respiratory phase of the cycle. Samples were taken at 4-min intervals through three complete cycles each from two independent cultures. A total of 68 RNA samples were isolated and analyzed. All RNA samples were analyzed for quality by using an Agilent Bioanalyzer capillary electrophoresis system. Thirty-two samples were selected from the best RNA profiles and aligned based solely on their phase position on the DO curve, before Affymetrix expression analysis. Sampling for flow cytometry is shown as red squares and for RNA as yellow triangles.