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. Author manuscript; available in PMC: 2012 Jun 8.
Published in final edited form as: Prog Brain Res. 2010;187:111–136. doi: 10.1016/B978-0-444-53613-6.00008-3

Figure 13.

Figure 13

Evidence for TRPM4 and TRPM5 channels in the preBötC. A) The mRNA coding for TRPM4 and TRPM5 is expressed in the preBötC region. Total RNA was extracted from preBötC and positive control tissues, and then reverse transcribed. Amplified products of the expected sizes were obtained for TRPM4 (301 bp), TRPM5 (483 bp) and GADPH (452 bp). Negative control reactions were performed without reverse transcriptase and amplified nothing. B) Immunohistochemistry from adult rats. Top panels (positive controls) show TRPM4 and TRPM5 in the compact nucleus ambiguous (cNA) taken from the ventral medulla immediately rostral to preBötC. Lower panels show TRPM4 and TRPM5 labeling in the preBötC at increasing magnification. Data in A were re-plotted with author permission from Crowder et al. (2007) J Physiol, 582, 1047–58.