Fig. 5.
Induced alterations in components of the p21WAF1 promoter-associated proteins. (A) Antibodies anti-HDAC1, anti-HDAC2, anti-Myc, anti-barrier-to-autointegration factor 155, anti-brm-related gene 1, anti-GCN5, anti-RNA polymerase II, anti-p300, and anti-Sp1 were used for ChIP assays. PCR amplification was done with p21WAF1 promoter TATA box region primers labeled with 32P (see Fig. 2). PCR products were analyzed as described in Experimental Procedures. ARP-1 cells were cultured without (–) and with (+)2μM SAHA for 3 h. Chromatin (0.05%)-extracted DNA (Input) was used for PCR amplification as chromatin loading. (B) ARP-1 cells were grown without (–) or with (+)2 μM SAHA for 3 h, and nuclear extracts were prepared. The level of each component was analyzed in Western blots by using the indicated antibodies.