Table 2.
Reproducibility of sphingolipid concentration measurements from human skeletal muscle.
| Intra-assay n=5 | Inter-assay n=5 | |||
|---|---|---|---|---|
| Compound | ng/100mg muscle | % CV | ng/100mg muscle | % CV |
| Sph | 12.5 | 0.4 | 12.6 | 1.0 |
| dhSph | 2.5 | 0.6 | 2.52 | 0.7 |
| S1P | 0.7 | 1.1 | 0.8 | 1.9 |
| C14-Cer | 3.7 | 0.4 | 3.7 | 1.0 |
| C16-Cer | 81.0 | 0.6 | 81.7 | 0.7 |
| C18:1-Cer | 147.0 | 0.3 | 147.5 | 0.8 |
| C18-Cer | 457.5 | 0.3 | 458.7 | 0.3 |
| C20-Cer | 29.8 | 0.8 | 30.0 | 0.7 |
| C24:1-Cer | 231.5 | 0.1 | 232.0 | 0.6 |
| C24-Cer | 298.5 | 0.5 | 300.0 | 0.6 |
The average (Ave) and coefficient of variation (CV) from 5 day replicate measures of sphingolipid concentrations from 5 human skeletal muscle samples are provided.