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Replicative vectors provide platforms for exogenous and ectopic expression in Thermococcus kodakarensis. T.k-E. coli shuttle vectors are the result of merging the entire Thermococcus nautilus-derived pTN1 sequence with a common E. coli plasmid (pCR2.1-Topo; Invitrogen). The marker additions provide the means to select T. kodakarensis transformants on any media, and the expression cassette provides a mechanism to ectopically express any gene of choice in T. kodakarensis.
