Table 2.
Colonization in target tissues obtained at necropsy after experimental challenge at midgestation with 107 CFU of B. abortus strain 2308
| Vaccination strategy | Log CFU/g (no. culture positive/total)a in: |
|||
|---|---|---|---|---|
| Parotid lymph node | Prescapular lymph node | Suprammamary lymph node | Placentome | |
| Hand RB51b | 0.8 ± 0.4 b (3/6)* | 0 ± 0 b (0/6)* | 0.7 ± 0.5 ab (2/6) | 4.0 ± 1.8 ab (3/6)* |
| Dart RB51c | 1.2 ± 0.5 b (4/7) | 0.3 ± 0.3 b (1/7)* | 0.9 ± 0.4 ab (4/7) | 4.5 ± 1.6 ab (4/7) |
| Booster RB51d | 0.8 ± 0.6 b (2/5)* | 0 ± 0 b (0/5)* | 0 ± 0 b (0/5)* | 1.7 ± 1.1 b (2/5)* |
| Control | 2.7 ± 0.3 a (6/6) | 1.7 ± 0.4 a (5/6) | 1.9 ± 0.5 a (5/6) | 7.6 ± 0.3 a (6/6) |
a
Data are means ± standard errors of the means. Colonization means with different letters are significantly different (P < 0.05). *, vaccination treatment group in which the incidence of infection in a tissue is significantly different (P < 0.05) from the incidence of infection in the control treatment group.
b
The vaccination dose was 1.1 × 1010 CFU.
c
The vaccination dose was 2.2 × 1010 CFU.
d
The initial vaccination dose was 1.1 × 1010 CFU, and the booster vaccination dose was 2.2 × 1010 CFU.