Fig 5.

Yeast biofilm adhesion assay. Microcultures arrayed on a polystyrene plate were grown in medium 13 with (A+ and B) or without (A−) supplemental arginine, histidine, and uridine for 92 h (A) or 63 h (B) at 30°C (A) or as indicated (B). Nonadherent cells were rinsed away, and the adherent cells were stained with crystal violet. Independent strains possessing wild-type (WT), anchor-negative (AM), and/or deleted (Δ) versions of Ywp1 were compared. The upper and lower portions of panel B show cultures that were identical except for their growth temperatures.