Fig 5.

Nuclear positions during unisexual reproduction in the wild type and kar7 mutant. Nuclei were visualized with the GFP-Nop1 protein. (A) In the wild-type XL280 strain, sexual hyphae are monokaryotic with unfused clamp connections. Two nuclei congress in the basidia and undergo karyogamy, meiosis occurs, and basidiospores are produced. DIC, differential interference contrast. (B) In contrast, in the kar7 mutants, the two nuclei congress but do not undergo karyogamy, resulting in a failure to produce the diploid nucleus or undergo meiosis, and consequently, no spore chains are produced. Scale bars = 5 μm.