Table 1.
Strain or plasmida | Serotype | Mating type | Genotype |
---|---|---|---|
Strains | |||
Wild type | |||
KN99α | A | α | Wild type |
KN99a | A | a | Wild type |
XL280 | D | α | Wild type |
JEC21 | D | α | Wild type |
JEC20 | D | a | Wild type |
MN142.3 | D | α | Diploid, ura5::NAT/ura5::NEO |
kar7 mutants | |||
SL2801 | A | α | KN99α kar7::NEO |
SL2811 | A | α | KN99α kar7::NEO |
SL2822 | A | α | KN99α kar7::NEO |
SL2832 | A | α | KN99α kar7::NEO |
SL2842 | A | α | KN99α kar7::NEO |
SL2851 | A | a | KN99akar7::NAT |
SL2861 | A | a | KN99akar7::NAT |
SL2871 | A | a | KN99akar7::NAT |
SL2882 | A | a | KN99akar7::NAT |
SL2892 | A | a | KN99akar7::NAT |
SL2903 | A | a | KN99akar7::NEO |
SL2913 | A | a | KN99akar7::NEO |
SL2923 | A | a | KN99akar7::NEO |
SL2933 | A | a | KN99akar7::NEO |
SL2943 | A | a | KN99akar7::NEO |
SL2761 | D | α | XL280 kar7::NEO |
SL2771 | D | α | XL280 kar7::NEO |
SL2782 | D | α | XL280 kar7::NEO |
SL2792 | D | α | XL280 kar7::NEO |
SL275 | D | α | JEC21 kar7::NEO |
SL3551 | D | α | MN142.3 kar7::HYG/KAR7 |
SL3561 | D | α | MN142.3 kar7::HYG/KAR7 |
SL3572 | D | α | MN142.3 kar7::HYG/KAR7 |
SL3582 | D | α | MN142.3 kar7::HYG/KAR7 |
SL3592 | D | α | MN142.3 kar7::HYG/KAR7 |
SL3602 | D | α | MN142.3 kar7::HYG/KAR7 |
SL3611 | D | α | SL355 kar7::HYG/kar7::URA5 ura5::NAT/ura5::NEO |
SL3622 | D | α | SL355 kar7::HYG/kar7::URA5 ura5::NAT/ura5::NEO |
SL3632 | D | α | SL355 kar7::HYG/kar7::URA5 ura5::NAT/ura5::NEO |
SL3643 | D | α | SL355 kar7::HYG/kar7::URA5 ura5::NAT/ura5::NEO |
SL3653 | D | α | SL355 kar7::HYG/kar7::URA5 ura5::NAT/ura5::NEO |
kar8 mutants | |||
SL2701 | D | α | XL280 kar8::NAT |
SL2742 | D | α | XL280 kar8::NAT |
SL271 | D | a | JEC20 kar8::NAT |
SL2721 | D | α | JEC21 kar8::NAT |
SL2732 | D | α | JEC21 kar8::NAT |
kar3 mutants | |||
SL2951 | A | α | KN99α kar3::NEO |
SL2962 | A | α | KN99α kar3::NEO |
SL2971 | A | a | KN99akar3::NAT |
SL2982 | A | a | KN99akar3::NAT |
kar4 mutants | |||
SL3661 | D | α | Diploid MN142.3 kar4::HYG/KAR4 ura5::NAT/ura5::NEO |
SL3671 | D | α | Diploid MN142.3 kar4::HYG/KAR4 ura5::NAT/ura5::NEO |
SL3682 | D | α | Diploid MN142.3 kar4::HYG/KAR4 ura5::NAT/ura5::NEO |
SL3721 | D | α | Haploid progeny of SL366 kar4::HYG |
SL3732 | D | α | Haploid progeny of SL366 kar4::HYG |
kar2 mutants | |||
SL3691 | D | α | Diploid MN142.3 kar2::HYG/KAR, ura5::NAT/ura5::NEO |
SL3701 | D | α | Diploid MN142.3 kar2::HYG/KAR2 ura5::NAT/ura5::NEO |
SL3712 | D | α | Diploid MN142.3 kar2::HYG/KAR2 ura5::NAT/ura5::NEO |
Strains with fluorescent Nop1 | |||
SL305 | A | α | KN99α with NOP1-mCherry (NEO) |
SL306 | A | α | KN99α with NOP1-mCherry(NEO) |
SL307 | A | α | KN99α with NOP1-mCherry(NEO) |
SL321 | A | a | KN99a with GFP-NOP1(NAT) |
SL322 | A | a | KN99a with GFP-NOP1(NAT) |
SL323 | A | a | KN99a with GFP-NOP1(NAT) |
SL324 | A | a | KN99a with GFP-NOP1(NAT) |
SL308 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
SL309 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
SL310 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
SL311 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
SL312 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
SL313 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
SL316 | A | a | SL290 (kar7) GFP-NOP1(NAT) |
SL317 | A | a | SL290 (kar7) GFP-NOP1(NAT) |
SL318 | A | a | SL290 (kar7) GFP-NOP1(NAT) |
SL319 | A | a | SL290 (kar7) GFP-NOP1(NAT) |
SL347 | A | α | SL280 (kar7) GFP-NOP1(NAT) |
SL348 | A | α | SL280 (kar7) GFP-NOP1 (NAT) |
SL349 | A | α | SL280 (kar7) GFP-NOP1(NAT) |
SL350 | A | α | SL280 (kar7) GFP-NOP1(NAT) |
SL338 | D | α | XL280 GFP-NOP1(NAT) |
SL339 | D | α | XL280 GFP-NOP1(NAT) |
SL340 | D | α | XL280 GFP-NOP1(NAT) |
SL341 | D | α | XL280 GFP-NOP1(NAT) |
SL342 | D | α | XL280 GFP-NOP1(NAT) |
SL380 | A | α | SL295 (kar3) GFP-NOP1(NAT) |
SL381 | A | α | SL295 (kar3) GFP-NOP1(NAT) |
SL383 | A | α | SL296 (kar3) GFP-NOP1(NAT) |
SL385 | A | α | SL296 (kar3) GFP-NOP1(NAT) |
SL374 | D | α | SL277 (kar7) GFP-NOP1(NAT) |
SL375 | D | α | SL277 (kar7) GFP-NOP1(NAT) |
SL376 | D | α | SL277 (kar7) GFP-NOP1(NAT) |
SL377 | D | α | SL277 (kar7) GFP-NOP1(NAT) |
Plasmids | |||
pSL01 | Ampr KanrNOP1 fragment in pCR21-TOPO for N-terminal GFP tagging with pCN19 | ||
pSL02 | Ampr KanrNOP1 fragment in pCR21-TOPO for N-terminal GFP tagging with pCN19 | ||
pSL04-02 | Ampr BglII fragment of NOP1 from pSL01 in pCN19/BamHI, vector for GFP-Nop1 for serotype A | ||
pSL05-1 | Ampr BglII fragment of NOP1 from pSL02 in pCN19/BamHI, vector for GFP-Nop1 for serotype D | ||
pCN19 | Ampr plasmid harboring GFP under histone H3 promoter |
Each superscript 1, 2, or 3 indicates a group of independent transformants or progeny.