Table 1.
Strains and plasmids used in this study
| Strain or plasmida | Serotype | Mating type | Genotype |
|---|---|---|---|
| Strains | |||
| Wild type | |||
| KN99α | A | α | Wild type |
| KN99a | A | a | Wild type |
| XL280 | D | α | Wild type |
| JEC21 | D | α | Wild type |
| JEC20 | D | a | Wild type |
| MN142.3 | D | α | Diploid, ura5::NAT/ura5::NEO |
| kar7 mutants | |||
| SL2801 | A | α | KN99α kar7::NEO |
| SL2811 | A | α | KN99α kar7::NEO |
| SL2822 | A | α | KN99α kar7::NEO |
| SL2832 | A | α | KN99α kar7::NEO |
| SL2842 | A | α | KN99α kar7::NEO |
| SL2851 | A | a | KN99akar7::NAT |
| SL2861 | A | a | KN99akar7::NAT |
| SL2871 | A | a | KN99akar7::NAT |
| SL2882 | A | a | KN99akar7::NAT |
| SL2892 | A | a | KN99akar7::NAT |
| SL2903 | A | a | KN99akar7::NEO |
| SL2913 | A | a | KN99akar7::NEO |
| SL2923 | A | a | KN99akar7::NEO |
| SL2933 | A | a | KN99akar7::NEO |
| SL2943 | A | a | KN99akar7::NEO |
| SL2761 | D | α | XL280 kar7::NEO |
| SL2771 | D | α | XL280 kar7::NEO |
| SL2782 | D | α | XL280 kar7::NEO |
| SL2792 | D | α | XL280 kar7::NEO |
| SL275 | D | α | JEC21 kar7::NEO |
| SL3551 | D | α | MN142.3 kar7::HYG/KAR7 |
| SL3561 | D | α | MN142.3 kar7::HYG/KAR7 |
| SL3572 | D | α | MN142.3 kar7::HYG/KAR7 |
| SL3582 | D | α | MN142.3 kar7::HYG/KAR7 |
| SL3592 | D | α | MN142.3 kar7::HYG/KAR7 |
| SL3602 | D | α | MN142.3 kar7::HYG/KAR7 |
| SL3611 | D | α | SL355 kar7::HYG/kar7::URA5 ura5::NAT/ura5::NEO |
| SL3622 | D | α | SL355 kar7::HYG/kar7::URA5 ura5::NAT/ura5::NEO |
| SL3632 | D | α | SL355 kar7::HYG/kar7::URA5 ura5::NAT/ura5::NEO |
| SL3643 | D | α | SL355 kar7::HYG/kar7::URA5 ura5::NAT/ura5::NEO |
| SL3653 | D | α | SL355 kar7::HYG/kar7::URA5 ura5::NAT/ura5::NEO |
| kar8 mutants | |||
| SL2701 | D | α | XL280 kar8::NAT |
| SL2742 | D | α | XL280 kar8::NAT |
| SL271 | D | a | JEC20 kar8::NAT |
| SL2721 | D | α | JEC21 kar8::NAT |
| SL2732 | D | α | JEC21 kar8::NAT |
| kar3 mutants | |||
| SL2951 | A | α | KN99α kar3::NEO |
| SL2962 | A | α | KN99α kar3::NEO |
| SL2971 | A | a | KN99akar3::NAT |
| SL2982 | A | a | KN99akar3::NAT |
| kar4 mutants | |||
| SL3661 | D | α | Diploid MN142.3 kar4::HYG/KAR4 ura5::NAT/ura5::NEO |
| SL3671 | D | α | Diploid MN142.3 kar4::HYG/KAR4 ura5::NAT/ura5::NEO |
| SL3682 | D | α | Diploid MN142.3 kar4::HYG/KAR4 ura5::NAT/ura5::NEO |
| SL3721 | D | α | Haploid progeny of SL366 kar4::HYG |
| SL3732 | D | α | Haploid progeny of SL366 kar4::HYG |
| kar2 mutants | |||
| SL3691 | D | α | Diploid MN142.3 kar2::HYG/KAR, ura5::NAT/ura5::NEO |
| SL3701 | D | α | Diploid MN142.3 kar2::HYG/KAR2 ura5::NAT/ura5::NEO |
| SL3712 | D | α | Diploid MN142.3 kar2::HYG/KAR2 ura5::NAT/ura5::NEO |
| Strains with fluorescent Nop1 | |||
| SL305 | A | α | KN99α with NOP1-mCherry (NEO) |
| SL306 | A | α | KN99α with NOP1-mCherry(NEO) |
| SL307 | A | α | KN99α with NOP1-mCherry(NEO) |
| SL321 | A | a | KN99a with GFP-NOP1(NAT) |
| SL322 | A | a | KN99a with GFP-NOP1(NAT) |
| SL323 | A | a | KN99a with GFP-NOP1(NAT) |
| SL324 | A | a | KN99a with GFP-NOP1(NAT) |
| SL308 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
| SL309 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
| SL310 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
| SL311 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
| SL312 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
| SL313 | A | a | SL285 (kar7) NOP1-mCherry(NEO) |
| SL316 | A | a | SL290 (kar7) GFP-NOP1(NAT) |
| SL317 | A | a | SL290 (kar7) GFP-NOP1(NAT) |
| SL318 | A | a | SL290 (kar7) GFP-NOP1(NAT) |
| SL319 | A | a | SL290 (kar7) GFP-NOP1(NAT) |
| SL347 | A | α | SL280 (kar7) GFP-NOP1(NAT) |
| SL348 | A | α | SL280 (kar7) GFP-NOP1 (NAT) |
| SL349 | A | α | SL280 (kar7) GFP-NOP1(NAT) |
| SL350 | A | α | SL280 (kar7) GFP-NOP1(NAT) |
| SL338 | D | α | XL280 GFP-NOP1(NAT) |
| SL339 | D | α | XL280 GFP-NOP1(NAT) |
| SL340 | D | α | XL280 GFP-NOP1(NAT) |
| SL341 | D | α | XL280 GFP-NOP1(NAT) |
| SL342 | D | α | XL280 GFP-NOP1(NAT) |
| SL380 | A | α | SL295 (kar3) GFP-NOP1(NAT) |
| SL381 | A | α | SL295 (kar3) GFP-NOP1(NAT) |
| SL383 | A | α | SL296 (kar3) GFP-NOP1(NAT) |
| SL385 | A | α | SL296 (kar3) GFP-NOP1(NAT) |
| SL374 | D | α | SL277 (kar7) GFP-NOP1(NAT) |
| SL375 | D | α | SL277 (kar7) GFP-NOP1(NAT) |
| SL376 | D | α | SL277 (kar7) GFP-NOP1(NAT) |
| SL377 | D | α | SL277 (kar7) GFP-NOP1(NAT) |
| Plasmids | |||
| pSL01 | Ampr KanrNOP1 fragment in pCR21-TOPO for N-terminal GFP tagging with pCN19 | ||
| pSL02 | Ampr KanrNOP1 fragment in pCR21-TOPO for N-terminal GFP tagging with pCN19 | ||
| pSL04-02 | Ampr BglII fragment of NOP1 from pSL01 in pCN19/BamHI, vector for GFP-Nop1 for serotype A | ||
| pSL05-1 | Ampr BglII fragment of NOP1 from pSL02 in pCN19/BamHI, vector for GFP-Nop1 for serotype D | ||
| pCN19 | Ampr plasmid harboring GFP under histone H3 promoter |
a
Each superscript 1, 2, or 3 indicates a group of independent transformants or progeny.