Table 1.
Plasmids used in this study
| Plasmid name | Sequence surrounding UAAA motifa | Description |
|---|---|---|
| pcat-UAAA | ...CACUUAAACAAU... | Parental plasmid described in Fig. 1 |
| pcat-GCCC | ...CACUGCCCCAAU... | Change of the four bases of the UAAA motif |
| pcat-GAAA | ...CACUGAAACAAU... | Change of the first base of the UAAA motif |
| pcat-UCAA | ...CACUUCAACAAU... | Change of the second base of the UAAA motif |
| pcat-UACA | ...CACUUACACAAU... | Change of the third base of the UAAA motif |
| pcat-UAAC | ...CACUUAACCAAU... | Change of the fourth base of the UAAA motif |
| pcat-GUAAA | ...CACGUAAACAAU... | Change of the upstream base adjacent to the UAAA motif |
| pcat-UAAAG | ...CACUUAAAGAAU... | Change of the downstream nucleotide adjacent to the UAAA motif |
| pcat-AAUAAA | .CUUCAAUAAACAAU... | Introduction of two A nucleotides to generate the eukaryotic polyadenylation signal AAUAAA |
| pcat-revUAAA | ...CACUUAAACAAU... | Revertant construct from pcat-UCAA |
| pcat-UAAA74 | ...UGUAAAUCACUUCAUUAAUAUUGUGUUUUAAUG... | Relocation of the UAAA motif 74 nt downstream from the stop codon in pcat-UCAA |
| pcat-UAAA118 | ...ACUAAAACGAUGUACCCUGAACAUUUCUUUUCCA... | Relocation of the UAAA motif 118 nt downstream from the stop codon in pcat-UCAA |
| pcat-UAAA118CS | ...ACUAAAACGAUGUACCCUGAACAUUUCAAUUCCA... | Addition of a cleavage site in the pcat-UAAA118 plasmid |
a
The UAAA motif is in bold. Underlining denotes a nucleotide changed from that in the parental plasmid UAAA.