Fig 2.

Enrichment of Hgl and Lgl in lipid rafts upon exposure to hRBCs is inhibited in the presence of galactose. Trophozoites (3.5 × 10⁶) were serum starved and exposed to 10 mM galactose (gal) or 10 mM mannose (man) prior to exposure to hRBCs. DRM was isolated and fractionated using sucrose gradient density centrifugation. Nineteen fractions and a pellet (20P) were collected and subjected to Western blot analysis using antibodies specific for Hgl (A), Lgl (B), or Igl (C). Average values and standard deviations for densitometric scans (n = 2) are reported as the percentage of total detergent-resistant protein for each subunit. The localization of Igl remained unchanged after exposure to galactose or mannose followed by hRBCs. The enrichment of Hgl and Lgl in lipid rafts after exposure to hRBCs was inhibited in the presence of galactose but not mannose.