Fig 6.

PIP₂ plays a role in Hgl and Lgl enrichment in lipid rafts. GFP-PH^BTK-expressing trophozoites (3.5 × 10⁶) were serum starved and exposed to hRBCs. DRM was isolated and fractionated using sucrose gradient density centrifugation. Nineteen fractions and a pellet (20P) were collected and subjected to Western blot analysis using antibodies specific for Hgl (A), Lgl (B), or Igl (C). Average values and standard deviations for densitometric scans (n = 2) are reported as the percentage of total detergent-resistant protein for each subunit. In the mutant, the submembrane distribution of the three subunits remained unchanged upon exposure to hRBCs. PIP₂ loading restored the enrichment of Hgl and Lgl in lipid raft fractions.