Fig 4.

Antibiotic production in ME mutants and the M145 strain of S. coelicolor. Growth of mutant strains on SMM-agar plates supplemented with 1% glucose (A) or plus the addition of 0.5% l-malate (B); plates were incubated for 7 days at 30°C. Strains used are as follows: 1, S. coelicolor M145; 2, TR5261; 3, TR2951; 4, TR2951c; 5, TRD; 6, TRDc5261; and 7, TRDc2951. Determination of antibiotics of the aforementioned strains grown in liquid medium SMM-Gluc for 72 h to detect actinorhodin (C) and for 60 h to detect undecylprodigiosin (D). Results represent the average of three independent experiments ± SD. Different letters denote statistical differences applying an ANOVA test using Sigma Stat 3.1 (P < 0.05).